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Reviews of "Integrated Single-Cell Atlases Reveal an Oral SARS-CoV-2 Infection and Transmission Axis"

Reviewers: Hongxiang Liu (University of Georgia), Zhonghou Wang, Wenxin Yu, Mohamed Ishan | 📗📗📗📗◻️ • Ophir Klein, Irit Miller Zmora (UCSF) | 📘📘📘📘📘

Published onJan 18, 2021
Reviews of "Integrated Single-Cell Atlases Reveal an Oral SARS-CoV-2 Infection and Transmission Axis"
key-enterThis Pub is a Review of
Integrated Single-Cell Atlases Reveal an Oral SARS-CoV-2 Infection and Transmission Axis
Description

ABSTRACT Despite signs of infection, the involvement of the oral cavity in COVID-19 is poorly understood. To address this, single-cell RNA sequencing data-sets were integrated from human minor salivary glands and gingiva to identify 11 epithelial, 7 mesenchymal, and 15 immune cell clusters. Analysis of SARS-CoV-2 viral entry factor expression showed enrichment in epithelia including the ducts and acini of the salivary glands and the suprabasal cells of the mucosae. COVID-19 autopsy tissues confirmed in vivo SARS-CoV-2 infection in the salivary glands and mucosa. Saliva from SARS-CoV-2-infected individuals harbored epithelial cells exhibiting ACE2 expression and SARS-CoV-2 RNA. Matched nasopharyngeal and saliva samples found distinct viral shedding dynamics and viral burden in saliva correlated with COVID-19 symptoms including taste loss. Upon recovery, this cohort exhibited salivary antibodies against SARS-CoV-2 proteins. Collectively, the oral cavity represents a robust site for COVID-19 infection and implicates saliva in viral transmission.

To read the original manuscript, click the link above.

Summary of Reviews: This pre-print uses single-cell RNA sequencing to investigate oral SARS-CoV-2 tropism and finds the mucosa represents a robust site of infection. Reviewers deem the study's claims reliable.

Reviewer 1 (Hongxiang Liu, Zhonghou Wang, Wenxin Yu, Mohamed Ishan) | 📗📗📗📗◻️

Reviewer 2 (Ophir Klein, Irit Miller Zmora) | 📘📘📘📘📘

RR:C19 Strength of Evidence Scale Key

📕 ◻️◻️◻️◻️ = Misleading

📙📙 ◻️◻️◻️ = Not Informative

📒📒📒 ◻️◻️ = Potentially Informative

📗📗📗📗◻️ = Reliable

📘📘📘📘📘 = Strong

To read the reviews, click the links below. 


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