Skip to main content
SearchLogin or Signup

Review 2: "Human organ chip-enabled pipeline to rapidly repurpose therapeutics during viral pandemics"

This study employs micro-fluidic devices to test candidate therapeutics blocking SARS-CoV-2 entry and demonstrates amodiaquine efficacy in preclinical models. The claims presented in this work are reliable but could be strengthened through more rigorous model characterization.

Published onSep 15, 2020
Review 2: "Human organ chip-enabled pipeline to rapidly repurpose therapeutics during viral pandemics"
1 of 2
key-enterThis Pub is a Review of
Human organ chip-enabled pipeline to rapidly repurpose therapeutics during viral pandemics
Human organ chip-enabled pipeline to rapidly repurpose therapeutics during viral pandemics

The rising threat of pandemic viruses, such as SARS-CoV-2, requires development of new preclinical discovery platforms that can more rapidly identify therapeutics that are active in vitro and also translate in vivo. Here we show that human organ-on-a-chip (Organ Chip) microfluidic culture devices lined by highly differentiated human primary lung airway epithelium and endothelium can be used to model virus entry, replication, strain-dependent virulence, host cytokine production, and recruitment of circulating immune cells in response to infection by respiratory viruses with great pandemic potential. We provide a first demonstration of drug repurposing by using oseltamivir in influenza A virus-infected organ chip cultures and show that co-administration of the approved anticoagulant drug, nafamostat, can double oseltamivir9s therapeutic time window. With the emergence of the COVID-19 pandemic, the Airway Chips were used to assess the inhibitory activities of approved drugs that showed inhibition in traditional cell culture assays only to find that most failed when tested in the Organ Chip platform. When administered in human Airway Chips under flow at a clinically relevant dose, one drug amodiaquine significantly inhibited infection by a pseudotyped SARS-CoV-2 virus. Proof of concept was provided by showing that amodiaquine and its active metabolite (desethylamodiaquine) also significantly reduce viral load in both direct infection and animal-to-animal transmission models of native SARS-CoV-2 infection in hamsters. These data highlight the value of Organ Chip technology as a more stringent and physiologically relevant platform for drug repurposing, and suggest that amodiaquine should be considered for future clinical testing.

RR:C19 Evidence Scale rating by reviewer:

  • Potentially informative. The main claims made are not strongly justified by the methods and data, but may yield some insight. The results and conclusions of the study may resemble those from the hypothetical ideal study, but there is substantial room for doubt. Decision-makers should consider this evidence only with a thorough understanding of its weaknesses, alongside other evidence and theory. Decision-makers should not consider this actionable, unless the weaknesses are clearly understood and there is other theory and evidence to further support it.



The preprint “Human organ chip-enabled pipeline to rapidly repurpose therapeutics during viral pandemics” by Si et al. reports on new results obtained from a host of preclinical models used to characterize infections with influenza A virus (IAV), pseudotyped spike protein-expressing viral particles (SARS-CoV-2pp) and SARS-CoV-2. These studies provide insights into mechanisms of infection and potential cooperative effects of various agents evaluated as candidate therapeutics for these respiratory viruses. Results show that oseltamivir in combination with nafamostat has beneficial effects on the therapeutic window for treatment of IAV relative to oseltamivir administration alone, although it is curious that studies are conducted with H1N1 rather than H3N2 strains.  Given reported differences in response in the literature, studies with oseltamivir treatment of both H1N1 and H3N2 strains in the airway chip devices would have been quite useful.   In addition, the anti-malarial drug amodiaquine is shown to be efficacious in reducing viral copy number in SARS-CoV-2pp-infected human lung airway chips, and in combination with its metabolite is likewise efficacious in reducing viral load in hamsters.  Multiple donor lots of human airway cells are used in these studies, but donor-to-donor differences are not explored in detail, yet represent another opportunity for future studies.  Overall, this is a very interesting study that holds much potential, but the mix of models including human airway epithelial cells in a microfluidic organ chip, Huh-7 cells in multiwell plates, and hamsters is a bit confusing, and inconsistent cross-correlation and validation lead to inconclusive findings.

From a model standpoint, several aspects of this work involving the human Lung Airway Chip are not completely clear. Which aspects of the small airway must be recapitulated is less clear ( than for prior work from this group (, in which ultrathin, cyclically stretched membranes separate alveolar type 2 (AT2) epithelial cells from microvascular endothelial cells. Here the role and physiological relevance of the endothelial layer and the epithelial-endothelial interactions is less clear.  Further, membrane properties and their role in governing epithelial and immune cell function is not clear. Is the pore size critical in ways beyond permissiveness to immune cell transport? How is the barrier function impacted? Permeability data is provided, but not using the standard airway Transepithelial Electrical Resistance (TEER) metric. Other questions relate to the throughput of these systems, and whether they can be scaled to higher replicate number like the Huh-7 multiwell plate systems.  At low throughput, are different conditions with regard to multiplicity of infection and drug concentration obtained across different devices or experiments, introducing additional potential uncertainties into the data? Are the fluid volumes and cell numbers in these devices critical? Does the flow in the endothelial chamber impart a fluid shear stress that is critical to the function of the model, and to the results obtained? Answers to these questions in the context of the human Lung Airway Chip model would be very helpful. Finally, extending studies to directly compare IAV to SARS-CoV-2 infection would provide insight towards pathogenesis and for potential benefit of nafamostat in treatment of COVID-19. Ideally such studies can be performed in the near future.

In terms of each model’s predictive behavior, direct comparison of drug efficacy against SARS-CoV-2 in the Lung Airway Chip versus animal models would be helpful. The antimalarial drugs chloroquine, hydroxychloroquine, and amodiaquine all show efficacy against SARS-CoV-2pp in Huh-7 cells. Of these three drugs, only amodiaquine is efficacious against SARS-CoV-2pp in the lung airway chip. Because pseudotyped virus assays only assess viral entry , not the full viral replication cycle, drug efficacies evaluations should be conducted in the context of SARS-CoV-2 infection. Of note, chloroquine been shown to be ineffective against SARS-CoV-2 in human lung epithelial cells expressing TMPRSS2 ( and has not shown benefit in most human clinical trials to date. Is amodiaquine superior in activity to chloroquine/hydroxychloroquine against SARS-CoV-2 in the lung airway chip? Why is amodiaquine effective in the hamster model but chloroquine/hydroxychloroquine is ineffective ( These considerations might lead to a mechanistic understanding of how amodiaquine works against SARS-CoV-2.

1 of 1

No comments here